Spectrum Chart
AlexaFluor 594
태그 보관물: dye
TRITC
Spectrum Chart
| Excitation (㎚) | Emission (㎚) |
| 555 | 580 |
Recommended Filter set
-
일반 형광 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| JNO-G(B) | 540/25 (527.5~552.5) |
565 (LP) | 605/55 (577.5~632.5) |
Narrow Excitation band, Bandpass Emission |
| 49004 | 545/25 (532.5~557.5) |
565 (LP) | 605/70 570~640) |
Narrow Excitation band, Bandpass Emission |
| 49305 | 546/22 (535~557) |
565 (LP) | 590/33 (573.5~606.5) |
Narrow Excitation band, Bandpass Emission |
-
레이저 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| 49908 | 532/10 (527~537) |
532 (LP) | 590/50 (565~615) |
For use with laser between 529~535㎚
Very Narrow Excitation band, |
| 49909 | 561/10 (556~566) |
561 (LP) | 600/50 (575~625) |
For use with laser between 557~563㎚
Narrow Excitation band, |
FITC
Spectrum Chart
| Excitation (㎚) | Emission (㎚) |
| 490 | 525 |
Recommended Filter
-
일반 형광 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| JNO-B(B) | 480/30 (465~495) |
505 (LP) | 535/40 (515~555) |
Medium Excitation band, Bandpass Emission |
| 49002 | 470/40 (450~490) |
495 (LP) | 525/50 (500~550) |
Wide Excitation band, Bandpass Emission |
| 49011 | 480/40 (460~500) |
510 (LP) | 535/50 (510~560) |
Wide Excitation band, Bandpass Emission |
-
레이저 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| 49904 | 488/10 (483~493) |
488 (LP) | 525/50 (500~550) |
For use with laser between 485~491㎚
Very Narrow Excitation band, |
Information
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
A succinimidyl-ester functional group attached to the fluorescein core, creating “NHS-fluorescein”, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.
FITC has excitation and emission spectrum peak wavelengths of approximately 495 ㎚ /519 ㎚. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.
DAPI
| Excitation (㎚) | Emission (㎚) |
| 359 | 461 |
Recommended Filter set
-
일반 형광 Lighting source
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| JNO-U(B) | 375/28 (361-389) |
415 (LP) | 460/50 (435-485) |
Narrow Excitation band, Bandpass Emission |
| 49000 | 350/50 (325-375) |
400 (LP) | 460/50 (435-485) |
Wide Excitation band, Bandpass Emission |
| 49028 | 395/25 (382.5-407.5) |
425 (LP) | 460/50 (435-485) |
For 395㎚ light sources
Narrow Excitation band, |
-
레이저 Lighting source -> 문의 주시기 바랍니다.
Information
DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.