tdTomato

 

Spectrum Chart

tdTomato Spectrum
Excitation () Emission ()
554 581

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)
565 (LP) 605/55
(577.5~632.5)
Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)
565 (LP) 605/70
570~640)
Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)
565 (LP) 590/33
(573.5~606.5)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)
532 (LP) 590/50
(565~615)
For use with laser between 529~535

Narrow Excitation band,
Longpass Emission

49909 561/10
(556~566)
561 (LP) 600/50
(575~625)
For use with laser between 557~563

Narrow Excitation band,
Bandpass Emission

 

Information

 

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 and 581 , respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.