카테고리 보관물: Fluorescence

Chroma, Filter, Fluorescence

49008

JNOPTIC Filter 49008

Download Catalog_49008

49008 Spectrum

Filters Type
ET560/40x EX
 T585lpxr BS
ET630/75m EM

 

– Filter set for Alexa Fluor 594, mCherry, Texas Red

Excitation (㎚) Emission (㎚)
590 617

Spectrum Comparison Fluor and 49008

Alexa Fluor 594 VS 49008

 

Best with these fluorochromes

 

49008 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Chroma, Filter, Fluorescence

49007

JNOPTIC Filter 49007

Download Catalog_49007

49007 Spectrum

Filters Type
ET710/75x EX
 T760lpxr BS
ET810/90m EM

 

– Filter set for Cy7

  • Main Fluorochromes : Cy7™
Excitation (㎚) Emission (㎚)
743 767

 

Spectrum Comparison Fluor and 49007

Cy7 VS 49007

 

Best with these fluorochromes

 

49007 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

49002

JNOPTIC Filter 49002

Download Catalog_49002

49002 Spectrum

Filters Type
ET470/40x EX
 T495lpxr BS
ET525/50m EM

 

– Filter set for EGFP (FITC/Cy2)

  • Main Fluorochromes : GFP
Excitation (㎚) Emission (㎚)
488 507

 

Spectrum Comparison Fluor and 49002

GFP VS 49002

 

Best with these fluorochromes

 

49002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

Chroma, Filter, Fluorescence

39009

JNOPTIC Filter 39009

Download Catalog_39009

39009 Spectrum

Filters Type
AT480/30x EX
 AT505DC BS
AT620/60m EM

 

– Filter set for Acridine Orange/Di-8-ANEPPS

  • Main Fluorochromes : EtBr
Excitation (㎚) Emission (㎚)
520 603

 

Spectrum Comparison Fluor and 39009

EtBr VS 39009

Best with these fluorochromes

  • Acridine Orange + RNA
  • Di-8-ANEPPS non-ratiometric
  • DiA
  • Ethidium Bromide
  • FM™ 1-43
  • LysoTracker Yellow HCK-123
  • Pulsar™ 650

 

39009 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

39003

JNOPTIC Filter 39003

Download Catalog_39003

39003 Spectrum

Filters Type
AT495/20x EX
 AT515DC BS
AT540/30m EM

 

– Filter set for EYFP/Venus/Citrine

  • Main Fluorochromes : YFP
Excitation (㎚) Emission (㎚)
514 527

 

Spectrum Comparison Fluor and 39003

YFP VS 39003

 

Best with these fluorochromes

 

39003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Chroma, Filter, Fluorescence

39001

JNOPTIC Filter 39001

Download Catalog_39001

39001 Spectrum

Filters Type
AT435/20x EX
 AT455DC BS
AT480/30m EM

 

– Filter set for ECFP/Cerulean

  • Main Fluorochromes : CFP
Excitation (㎚) Emission (㎚)
433 475

 

Spectrum Comparison Fluor and 39001

CFP VS 39001

 

Best with these fluorochromes

  • AmCyan
  • Atto 425
  • Cerulean
  • CFP
  • ECFP

 

39001 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Fluorescence, Fluorochrome

Cy5.5

Spectrum Chart

Cy5.5 Spectrum
Excitation (㎚) Emission (㎚)
675 695

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49022 545/25
(532.5~557.5)		 565 (LP) 605/70
(570~640)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49914 642/20
(632~652)		 647 (LP) 705/72
(669~741)		 For use with laser between 633~650㎚

Narrow Excitation band,
Bandpass Emission

 

Information

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Cyanine 5.5 (Cy5.5) 염료는 형광 이미징 및 기타 형광 기반 생화학 분석을 위해 생물 분자를 표지하는 데 사용되었습니다. 그들은 펩타이드, 단백질 및 올리고 제 등의 라벨링에 널리 사용됩니다. Cy5.5 염료는 가장 일반적인 적색 형광체의 한 유형입니다.

 

 

Fluorescence, Fluorochrome

tdTomato

 

Spectrum Chart

tdTomato Spectrum
Excitation (㎚) Emission (㎚)
554 581

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Longpass Emission
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

 

Information

 

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 ㎚ and 581 ㎚, respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

 

 

 

Fluorescence, Fluorochrome

YFP

Spectrum Chart

YFP Spectrum
EX(㎚) EM(㎚)
514 527

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39003 495/20
(485~505)		 515 (LP) 535/30
(520~550)		 Narrow Excitation band,
Bandpass Emission
49003 500/20
(490~510)		 515 (LP) 540/30
(525~555)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49905 514/10
(509~519)		 514 (LP) 545/40
(525~565)		 For use with laser between 511~517㎚

Narrow Excitation band,
Bandpass Emission

 

 

Information

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 ㎚ and its emission peak is 527 ㎚. Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.

노란색 형광 단백질 (YFP)은 원래 해파리 Aequorea victoria에서 파생 된 녹색 형광 단백질 (GFP)의 유전 적 돌연변이입니다. 그것의 여기 피크는 514 ㎚이고 방출 피크는 527 ㎚ 입니다. 부모 GFP와 마찬가지로 YFP는 형광 현미경 검사에 유용한 그 특성 덕분에 세포 및 분자 생물학에서 유용한 도구입니다.

 

Fluorescence, Fluorochrome

GFP

댓글 한 개

 

Spectrum chart

GFP Spectrum
Excitation (㎚) Emission (㎚)
488 507

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)		 505 (LP) 535/40
(515~555)		 Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)		 495 (LP) 525/50
(500~550)		 Wide Excitation band,
Bandpass Emission
49020 480/20
(470~490)		 495 (LP) 510/20
(500~530)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)		 488 (LP) 525/50
(500~550)		 For use with laser between 485~491㎚

Narrow Excitation band,
Bandpass Emission

 

 

Information

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 ㎚ and a minor one at 475 ㎚. Its emission peak is at 509 ㎚, which is in the lower green portion of the visible spectrum.

녹색 형광 단백질 (GFP)은 238 아미노산 잔기 (26.9 kDa)로 구성된 단백질로 청색에서 자외선까지의 빛에 노출되면 밝은 녹색 형광을 나타냅니다. 많은 다른 해양 생물이 유사한 녹색 형광 단백질을 가지고 있지만, GFP는 전통적으로 해파리 Aequorea victoria에서 먼저 분리 된 단백질을 의미합니다. A. 빅토리아의 GFP는 파장 395 ㎚ 에서 주요한 여기 피크와 475 ㎚ 에서 사소한 피크를 나타내고 그 방출 피크는 가시 광선 스펙트럼의 낮은 녹색 부분에 있는 509 ㎚ 입니다.