카테고리 보관물: Fluorescence

AlexaFluor 514

Spectrum Chart

AlexaFluor 514 Spectrum

Excitation () Emission ()
517 539

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39003 495/20
(485~505)
515 (LP) 535/30
(520~550)
Narrow Excitation band,
Bandpass Emission
49003 500/20
(490~510)
515 (LP) 540/30
(525~555)
Narrow Excitation band,
Bandpass Emission
49023 480/40
(460~500)
510 (LP) 535/50
(510~560)
Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source  사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49905 514/10
(509~519)
514 (LP) 545/40
(525~565)
For use with laser between 511~517

Narrow Excitation band,
Bandpass Emission

49906 514/10
(509~519)
514 (LP) 570/50
(545~595)
For use with laser between 511~517

Narrow Excitation band,
Bandpass Emission

 

 

 

AlexaFluor 488

Spectrum Chart

AlexaFluor 488 Spectrum
Excitation () Emission ()
498 520

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)
505 (LP) 535/40
(515~555)
Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)
495 (LP) 525/50
(500~550)
Wide Excitation band,
Bandpass Emission
49011 480/40
(460~500)
510 (LP) 535/50
(510~560)
Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)
488 (LP) 525/50
(500~550)
For use with laser between 485~491

Narrow Excitation band,
Bandpass Emission

 

 

49910

JNOPTIC Filter 49910

Download Catalog_49910

49910 Spectrum

Filters Type
ZET561/10x EX
 ZT514rdc BS
ET630/70m EM

 

– Filter set of 561nm Laser Bandpass Set for AlexaFluor 568, AlexaFluor 594, Texas Red®, mCherry

For use with lasers between 557-563

Excitation () Emission ()
587 610

 

Spectrum Comparison Fluor and 49910

mCherry VS 49910

 

49910 Spec

  • Typical Application(s) : Laser (Non-TIRF)
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

49915

JNOPTIC Filter 49915

Download Catalog_49915

49915 Spectrum

Filters Type
ZET365/20x EX
 ZT365bcm BS
ET425lp EM

 

– Filter set of 355-375nm Laser Longpass Set for Uncaging and Ablation

Excitation () Emission ()
346 442

 

Spectrum Comparison Fluor and 49915

Alexa Fluor 350 VS 49915

 

49915 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26×38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

AlexaFluor 350

Spectrum Chart

AlexaFluor 350 Spectrum
Excitation () Emission ()
346 442

 

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-U(B) 375/28
(361-389)
415 (LP) 460/50
(435-485)
Narrow Excitation band,
Bandpass Emission
49000 350/50
(325-375)
400 (LP) 460/50
(435-485)
Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source  사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49915 365/20
(355~375)
365 (LP) 425 (LP) Narrow Excitation band,
longpass Emission

 

 

Acridine Orange+DNA

Spectrum Chart

Acridine Orange+DNA Spectrum
Excitation () Emission ()
500 526

 


Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)
505 (LP) 535/40
(515~555)
Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)
495 (LP) 525/50
(500~550)
Wide Excitation band,
Bandpass Emission
49011 480/40
(460~500)
510 (LP) 535/50
(510~560)
Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

19004

JNOPTIC Filter 19004

Download Catalog_19004

19004 Spectrum

Filters Type
AT540/25x EX
AT565DC BS
AT575lp EM

 

– Filter set for TRITC/Cy3 Longpass

  • Main Fluorochromes : FITC
Excitation () Emission ()
555 580

 

Spectrum Comparison Fluor and 19004

TRITC VS 19004

 

  • R-phycoerythrin

 

19004 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

 

19002

JNOPTIC Filter 19002

Download Catalog_19002

19002 Spectrum

Filters Type
AT480/30x EX
 AT505DC BS
AT515lp EM

 

– Filter set for GFP/FITC Longpass

  • Main Fluorochromes : FITC
Excitation () Emission ()
490 525

 

Spectrum Comparison Fluor and 19002

FITC VS 19002

 

  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

 

19002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

19000

JNOPTIC Filter 19000

Download Catalog_19000

19000 Spectrum

Filters Type
AT375/28x EX
AT415DC BS
AT435lp EM

– Filter set for UV/DAPI Longpass

  • Main Fluorochromes : DAPI
Excitation () Emission ()
359 461

 

Spectrum Comparison Fluor and 19000

DAPI VS 19000

Best with these fluorochromes

  • Calcofluor White
  • DAPI

 

19000 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes