카테고리 보관물: Fluorescence

Chroma, Fluorescence

qpcr Filter Set

quantitative real time Polymerase Chain Reaction

실시간 중합효소 연쇄반응

PCR을 진행하는데 있어서 형광물질을 사용하여 관측되는 DNA나 RNA의 양을 정확하게 측정하는 방법.

목표 DNA 분자의 증폭과 양의 측정을 동시에 시행한다.

핵산(DNA, RNA)을 다양한 시약으로 착색시켜 qPCR에서 형광 검출이 용이하게 특화된 필터를 공급합니다.

적용가능한 채널

FAM / SYBR Green Channel

HEX / VIC / JOE Channel

Cy3 Channel

Texas Red / ROX Channel

Cy5 / Q670 Channel

Cy5.5 / Qusar 705 Channel

Chroma’s ET sputtered filters are durable hard-coated filters, won’t “burn out” or delaminate, and are optimized to provide the best combination of Contrast, Brightness, Color Seperation, Resistration.

Filter, Fluorescence

Mycobacterium tuberculosis

결핵 검진을 위한 형광현미경 검경법

Auramine O나 Auramine-rhodamine을 이용한 염색법으로 20x 또는 40x 대물렌즈로 관찰 한다 (접안렌즈 기준 200배 또는 400배). 이러한 형광염색은 Ziehl-Neelsen (ZN) 염색에 비해 민감도가 10% 높아서 관찰량이 많은 사용자에게 권고하는 것이 좋다.

JNO-19008

Ex: 425~475 ㎚, Dm: 485 ㎚, Em: 495 ㎚~
Auramine Longpass
Olympus Filter cube
Chroma, Filter

Industrial Filters

UV FILTER ( 자외선 투과 필터 )

270 ㎚ 투과필터 ( 263 ~ 277 ㎚ ) 350 ㎚ 투과필터( 325 ~ 375 ㎚ )

NIR FILTER ( 근적외선 필터 )

780 ㎚ 이상 투과필터 ( 780 ㎚ ~ ) 밴드투과 필터 ( 771 ~ 851 ㎚ )

Near Infrared Camera
근적외선 관찰 카메라

AcquCAM 23GR2

칼라 카메라

– 근적외선 대응 – 		Color Camera

UV 자극을 이용한 형광 이미지 _ DAPI
AcquCAM 23S2

흑백 카메라

– 근적외선 대응 –		 Monochrome Camera ( IR-DIC Imagea )

적외선을 이용한 미분 간섭 관찰 이미지

Filter, Fluorescence

79004

JNOPTIC Filter 79004

ET – Fura 2/GFP

79004 Spectrum

Filters Type
ET340x EX
ET380x EX
ET470/40x EX
T495lpxru BS
ET535/70m EM

 

79004 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

 

Filter, Fluorescence

79003

JNOPTIC Filter 79003

ET – Fura 2/TRITC

79003 Spectrum

Filters Type
ET340x EX
ET380x EX
ET555/25x EX
79003bs BS
79003m EM

 

79003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

Filter, Fluorescence

79001

JNOPTIC Filter 79001

ET – Fura 2

79001 Spectrum

Filters Type
ET340x EX
ET380x EX
T400lp BS
ET510/80m EM

 

79001 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

 

Filter, Fluorescence

89403

JNOPTIC Filter 89403

ET – 436-28/506-21/578-24/730-40 Multi LED set

Quad band catalog set for Multi LED light engines like those manufactured by Lumencor, CoolLED, Excelitas and Cairn Research. Excitation passbands are matched to discrete, triggerable LEDs appropriate for excitation of CFP/YFP/RFP/Cy7 and similar fluors. Not intended for use with white light sources.

89403 Spectrum

Filters Type
89403x EX
89403bs BS
89403m EM

 

89403 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters.

 

Filter, Fluorescence

59217

JNOPTIC Filter 59217

ET – ECFP/EYFP with single band exciters

59217 Spectrum

Filters Type
ET430/24x EX
ET500/20x EX
69008bs  BS
59017m EM

 

59217 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

 

Filter, Fluorescence

59222

JNOPTIC Filter 59222

ET – EGFP/mCherry (or FITC/TxRed) with single band exciters

59222 Spectrum

Filters Type
ET470/40x EX
ET572/35x EX
59022bs  BS
59022m EM

 

59222 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

 

Filter, Fluorescence

59017

JNOPTIC Filter 59017

ET – ECFP/EYFP

59017 Spectrum

Filters Type
59017x EX
69008bs BS
59017m EM

 

59017 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.