The Prime BSI back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. The Prime BSI combines 95% quantum efficiency with a low 1.0 e- read noise to deliver the most sensitive camera based on sCMOS technology at over 40 frames per second.
Resolution and Pixel Size
The Prime BSI features 6.5 μm pixels, the accepted standard for most live cell applications using 40x and 60x magnification. This pixel size provides highly detailed images across the imaging plane and is most suitable for the broadest range of microscope objectives.
Superior Background Quality
The Prime BSI features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern free images with minimal pixel defects, delivering improved image quality in low light conditions.
Advanced Triggering
Programmable Scan Mode provides increased control over the rolling shutter exposure and read-out functionality of CMOS sensors for applications that require control over the line time. Sequenced Multiple Acquisition Real Time Streaming (SMART) Streaming enables the Prime BSI to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.
Advanced Features
The Prime BSI offers PrimeEnhance active denoising which provides a real-time quantitative increase in the signal to noise ratio to maximize sensitivity at ultra-low light levels.
UV(200-400nm), Visible(400-700nm), Near Infrared(700-1000nm)
Read Noise(median)
1.6 e-
Sensor Area
13.2mm X 13.2mm
Frame Rate
41fps @ 16bit / 82fps @12bit
Prime 95B
1.44M pixels (1200×1200)
Running a Prime 95B Scientific CMOS camera in Olympus CellSens Software
Sensitivity
The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.
Advanced Features
The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI. PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications. PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.
Live Particle Tracking in Ocular / Live particle tracking from Photometrics with live statistics. Use live particle tracking to acquire better data – determine whether molecules are behaving as expected before time-consuming data acquisition.
Advanced Triggering
Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times. The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.
The brand new Kinetix family of back-illuminated sCMOS cameras delivers a framerate and field of view unmatched by any other sCMOS camera. KINETIX CAMERA
● PrimeEnhance™ quantitatively increases the Signal to Noise Ratio by 3X-5X, increasing the clarity and quality of images.
● PrimeLocate™ dynamically evaluates acquired images and reduces the surplus of data generated during high speed super-resolution imaging.
<Application>
■ Single Molecule Detection
■ High-Sensitive – Calcium Ratio, Live Cell
■ Large Area Image
Specification
Sensor Technology
sCMOS
Quantum Efficiency
95%
Pixel Size
11um X 11um
Supported Interfaces
PCIe and USB3.0
Spectrum Capabilities
UV(200-400nm), Visible(400-700nm), Near Infrared(700-1000nm)
Read Noise(median)
1.6 e-
Sensor Area
13.2mm X 13.2mm
Frame Rate
41fps @ 16bit / 82fps @12bit
Prime 95B
1.44M pixels
Sensitivity
The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.
Advanced Features
The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI. PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications. PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.
Advanced Triggering
Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times. The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.
